Identification of a three-gene model as a powerful diagnostic tool for the recognition of ALK negative ALCL

Agnelli, L.; Mereu, E.; Pellegrino, E.; Limongi, T.; Kwee, I.; Bergaggio, E.; Ponzoni, M.; Zamo', Alberto; Iqbal, J.; Piccaluga, P. P.; Neri, A.; Chan, J. C.; Pileri, S.; Bertoni, F.; Inghirami, G.; Piva, R.

Anaplastic Large Cell Lymphomas (ALCL) are a clinically and biologically heterogeneous disease including the ALK+ and ALK- systemic forms. While ALK+ ALCL are molecularly characterized and can be readily diagnosed, specific immunophenotypic or genetic features to define ALK- ALCL are missing, and their distinction from other T-Cell Non- Hodgkin Lymphomas (T-NHL) remains controversial. Here, we undertook a transcriptional profiling meta-analysis of 309 cases, including ALCL and other primary T-NHL samples. Pathway discovery and prediction analyses defined a minimum set of genes capable to recognize ALK- ALCL. Application of RT-qPCR in independent data sets from cryopreserved and formalin-fixed paraffin embedded (FFPE) samples validated a three-gene model (TNFRSF8, BATF3, TMOD1) able to successfully separate ALK- ALCL from PTCLNOS, with overall accuracy near 97%. In conclusion, our data justify the possibility to translate RT-qPCR protocols to routine clinical settings as a new approach to objectively dissect T-NHL and to select more appropriate therapeutic protocols.

Titolo:	Identification of a three-gene model as a powerful diagnostic tool for the recognition of ALK negative ALCL
Autori:	Agnelli L. Mereu E. Pellegrino E. Limongi T. Kwee I. Bergaggio E. Ponzoni M. ZAMO', Alberto Iqbal J. Piccaluga P. P. Neri A. Chan J. C. Pileri S. Bertoni F. Inghirami G. Piva R. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2012
Rivista:	BLOOD
Abstract:	Anaplastic Large Cell Lymphomas (ALCL) are a clinically and biologically heterogeneous disease including the ALK+ and ALK- systemic forms. While ALK+ ALCL are molecularly characterized and can be readily diagnosed, specific immunophenotypic or genetic features to define ALK- ALCL are missing, and their distinction from other T-Cell Non- Hodgkin Lymphomas (T-NHL) remains controversial. Here, we undertook a transcriptional profiling meta-analysis of 309 cases, including ALCL and other primary T-NHL samples. Pathway discovery and prediction analyses defined a minimum set of genes capable to recognize ALK- ALCL. Application of RT-qPCR in independent data sets from cryopreserved and formalin-fixed paraffin embedded (FFPE) samples validated a three-gene model (TNFRSF8, BATF3, TMOD1) able to successfully separate ALK- ALCL from PTCLNOS, with overall accuracy near 97%. In conclusion, our data justify the possibility to translate RT-qPCR protocols to routine clinical settings as a new approach to objectively dissect T-NHL and to select more appropriate therapeutic protocols.
Handle:	http://hdl.handle.net/11562/429974
Appare nelle tipologie:	01.01 Articolo in Rivista

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