Background: There is little information on temperature and period of freezing for generating hemolysate and blood cell lysate. Materials and methods: Primary tubes containing whole blood were frozen at -20 °C and -80 °C and then serially thawed to assess cell lysis and clinical chemistry tests in centrifuged plasma. Results and conclusions: Massive amount of blood cells lysis could only be obtained by storing samples 12 h at -20 °C, or 2 h at -80 °C. © 2011 The Canadian Society of Clinical Chemists.
Influence of temperature and period of freezing on the generation of hemolysate and blood cell lysate.
LIPPI, Giuseppe;
2011-01-01
Abstract
Background: There is little information on temperature and period of freezing for generating hemolysate and blood cell lysate. Materials and methods: Primary tubes containing whole blood were frozen at -20 °C and -80 °C and then serially thawed to assess cell lysis and clinical chemistry tests in centrifuged plasma. Results and conclusions: Massive amount of blood cells lysis could only be obtained by storing samples 12 h at -20 °C, or 2 h at -80 °C. © 2011 The Canadian Society of Clinical Chemists.
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