SV 40 nucleoprotein complexes (NPC) containing viral DNA and cellular histones were extracted from nuclei of permissive cells and treated with either EcoR1 or BamI endonucleases. The fraction of SV 40 linear NPC, monitored by electron microscopy, reached a plateau value of about 15-20% after one hour and no further change occurred during further incubation for 2 hours. Free viral DNA added to the incubation mixture was totally cleaved. During the incubations of NPC and DNA, no redistribution of histones occurred, all the complexe still contained on average 21 nucleosomes and no nucleosomes were generated on the naked viral DNA. Our results suggest a random location and absence of movement of the nucleosomes in vitro on SV 40 nucleoprotein complex isolated from infected cells. © 1976.
Random location and absence of movement of the nucleosomes on SV 40 nucleoprotein complex isolated from infected cells
PIGNATTI, Pierfranco;
1976-01-01
Abstract
SV 40 nucleoprotein complexes (NPC) containing viral DNA and cellular histones were extracted from nuclei of permissive cells and treated with either EcoR1 or BamI endonucleases. The fraction of SV 40 linear NPC, monitored by electron microscopy, reached a plateau value of about 15-20% after one hour and no further change occurred during further incubation for 2 hours. Free viral DNA added to the incubation mixture was totally cleaved. During the incubations of NPC and DNA, no redistribution of histones occurred, all the complexe still contained on average 21 nucleosomes and no nucleosomes were generated on the naked viral DNA. Our results suggest a random location and absence of movement of the nucleosomes in vitro on SV 40 nucleoprotein complex isolated from infected cells. © 1976.
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