In this study, we report on the remarkable two-photon excited fluorescence efficiency in the "biological window" of CaF2:Tm3+,Yb3+ nanoparticles. Based on the strong Tm3+ ion emission (at around 800 nm), tissue penetration depths as large as 2 mm have been demonstrated, which are more than four times those achievable based on the visible emissions in comparable CaF2:Er3+,Yb3+ nanoparticles. The outstanding penetration depth, together with the fluorescence thermal sensitivity demonstrated here, makes CaF2:Tm3+,Yb3+ nanoparticles ideal candidates as multifunctional nanoprobes for high contrast and highly penetrating in vivo fluorescence imaging applications.

NIR-to-NIR two-photon excited CaF2:Tm3+,Yb3+ nanoparticles: multifunctional nanoprobes for highly penetrating fluorescence bio-imaging.

PEDRONI, Marco;PICCINELLI, FABIO;CONTI, GIAMAICA;SBARBATI, Andrea;BETTINELLI, Marco Giovanni;SPEGHINI, Adolfo
2011-01-01

Abstract

In this study, we report on the remarkable two-photon excited fluorescence efficiency in the "biological window" of CaF2:Tm3+,Yb3+ nanoparticles. Based on the strong Tm3+ ion emission (at around 800 nm), tissue penetration depths as large as 2 mm have been demonstrated, which are more than four times those achievable based on the visible emissions in comparable CaF2:Er3+,Yb3+ nanoparticles. The outstanding penetration depth, together with the fluorescence thermal sensitivity demonstrated here, makes CaF2:Tm3+,Yb3+ nanoparticles ideal candidates as multifunctional nanoprobes for high contrast and highly penetrating in vivo fluorescence imaging applications.
multifunctional nanoprobes; fluorescence bio-imaging; nanothermometers; near-infrared upconversion; calcium fluoride
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/366595
Citazioni
  • ???jsp.display-item.citation.pmc??? 30
  • Scopus 349
  • ???jsp.display-item.citation.isi??? 340
social impact