Analytical performances of the D-dimer assay for the immulite 2000 automated immunoassay analyser

We evaluated the analytical performances of a new commercial chemiluminescent enzyme immunometric assay for d-dimer measurement on the Immulite 2000 automated analyser. The within- and between-run coefficients of variations for low, intermediate and high d-dimer concentrations ranged from 2.2% to 5.1% and from 2.9% to 6.0% respectively. The assay was proven linear in a range of d-dimer concentrations comprised between 324 and 7602 ng/ml. Comparison of samples collected in either 0.109 mol/l sodium citrate or lithium-heparin tubes by Bland-Altman plots and nonparametric regression analysis (y = 0.858x - 39, r = 0.997; P < 0.0001) revealed a minimum bias, almost completely attributable to the dilution effect of the anticoagulant. Results of 56 outpatients' citrated plasma samples analysed with the Immulite 2000 d-dimer assay were compared with those of the current reference commercial immunoassay on the Mini Vidas Immunoanalyser. Although the nonparametric regression according to the met...