Background: The techniques universally believed as most reliable for determination of Carbohydrate-Deficient Transferrin (CDT) are HPLC andcapillary electrophoresis (CE). Recently a reagent kit for CDT analysis to be used in a multicapillary electropherograph has been introduced. Thepresent work was aimed at a validation of this new commercial system by application of the analytical chemistry parameters and by comparisonwith validated CE and HPLC methods.Methods: One hundred forty serum samples were analyzed with multicapillary CE (Capillarys™, Sebia, France) using kit reagents(CAPILLARYS™ CDT assay), with single capillary electropherograph (P/ACE MDQ, Beckman Coulter, USA) using an original methoddeveloped by our group and with HPLC, performed on a gradient HPLC (Shimadzu Europe, Germany), using column and reagents provided in kit(ClinRep®, Recipe, Germany).Results: The separation efficiency of the multicapillary system was about 15,000 theoretical plates/column. The resolution of the transferrinisoforms ranged from 1.23 to 1.67. The variation coefficient was b10% in both intra-run and between run tests. The correlation of results ofmulticapillary CE with those obtained with single CE and HPLC was statistically significant.Conclusions: The multicapillary system shows high productivity with good analytical performances; however, a confirmation of positive resultswith an alternative method is required.
Fully automated analysis of carbohydrate-deficient transferrin (CDT) by using a multicapillaryelectrophoresis system
BORTOLOTTI, Federica;DE PAOLI, Giorgia;PASCALI, Jennifer;TAGLIARO, Franco
2007-01-01
Abstract
Background: The techniques universally believed as most reliable for determination of Carbohydrate-Deficient Transferrin (CDT) are HPLC andcapillary electrophoresis (CE). Recently a reagent kit for CDT analysis to be used in a multicapillary electropherograph has been introduced. Thepresent work was aimed at a validation of this new commercial system by application of the analytical chemistry parameters and by comparisonwith validated CE and HPLC methods.Methods: One hundred forty serum samples were analyzed with multicapillary CE (Capillarys™, Sebia, France) using kit reagents(CAPILLARYS™ CDT assay), with single capillary electropherograph (P/ACE MDQ, Beckman Coulter, USA) using an original methoddeveloped by our group and with HPLC, performed on a gradient HPLC (Shimadzu Europe, Germany), using column and reagents provided in kit(ClinRep®, Recipe, Germany).Results: The separation efficiency of the multicapillary system was about 15,000 theoretical plates/column. The resolution of the transferrinisoforms ranged from 1.23 to 1.67. The variation coefficient was b10% in both intra-run and between run tests. The correlation of results ofmulticapillary CE with those obtained with single CE and HPLC was statistically significant.Conclusions: The multicapillary system shows high productivity with good analytical performances; however, a confirmation of positive resultswith an alternative method is required.
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