The protein population secreted from three wine-contaminant microorganisms was studied by two-dimensional electrophoresis and screened for proteases. Proteolytic enzymes were electrophoretically purified and their activity, optimum pH and temperature determined. The protease of Acetobacter aceti maintained its activity over the range of pHs 3.0- 5.0, thus being of potential biotechnological interest.

Acidic extracellular proteases from microrganisms of fairly acidic niche.

BOSSI, Alessandra Maria;ZAPPAROLI, Giacomo
2006

Abstract

The protein population secreted from three wine-contaminant microorganisms was studied by two-dimensional electrophoresis and screened for proteases. Proteolytic enzymes were electrophoretically purified and their activity, optimum pH and temperature determined. The protease of Acetobacter aceti maintained its activity over the range of pHs 3.0- 5.0, thus being of potential biotechnological interest.
Lactobacillus hilgardii; Acetobacter aceti; Brettanomyces bruxellensis; extracellular protease; two-dimensional electrophoresis; zymogram
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/308048
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