In the human glioblastoma cell line U87, the activity of serine racemase (SR), catalyzing the isomerisation of serine, was inversely regulated by D-serine and nitric oxide (NO), a neuromodulator and a neurotransmitter, respectively. SR activity was dose-dependently enhanced up to five times in cells treated with 10 MM D-serine, whereas it was inhibited by NO. Further-more, D-serine was found to induce the denitrosylation of SR purified from mouse brain. These results suggest that serine racemase activity in astrocyte is regulated inversely by D-serine and NO. SR should be inhibited through nitrosylation by NO and activated through denitrosylation elicited by D-serine. (C) 2005 Elsevier Ireland Ltd. All fights reserved.
Regulation of serine racemase activity by d-serine and nitric oxide in human glioblastoma cells.
MARIOTTO, Sofia Giovanna;SUZUKI, Hisanori
2006-01-01
Abstract
In the human glioblastoma cell line U87, the activity of serine racemase (SR), catalyzing the isomerisation of serine, was inversely regulated by D-serine and nitric oxide (NO), a neuromodulator and a neurotransmitter, respectively. SR activity was dose-dependently enhanced up to five times in cells treated with 10 MM D-serine, whereas it was inhibited by NO. Further-more, D-serine was found to induce the denitrosylation of SR purified from mouse brain. These results suggest that serine racemase activity in astrocyte is regulated inversely by D-serine and NO. SR should be inhibited through nitrosylation by NO and activated through denitrosylation elicited by D-serine. (C) 2005 Elsevier Ireland Ltd. All fights reserved.
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