Pro-apoptotic effect of maize lipid transfer protein on mammalian mitochondria

Crimi, Massimo; Astegno, Alessandra; Zoccatelli, Gianni; Degli Esposti, M.

doi:10.1016/j.abb.2005.10.024

To assess the effect of lipids and lipid exchange in the pro-apoptotic release of cytochrome c, we investigated the ability of a plant lipid transfer protein (LTP) to initiate the apoptotic cascade at the mitochondrial level. The results show that maize LTP is able to induce cytochrome c release from the intermembrane space of mouse liver mitochondria without significant mitochondrial swelling, similarly to mouse full-length Bid. This effect is influenced by the presence of specific lipids, since addition of lysolipids like lysophosphatidylcholine strongly stimulates the LTP-induced release of cytochrome c while it is inhibited by removal of endogenous free lipids with a complete suppression of the LTP-induced release of cytochrome c. The results are discussed in light of the possible role of lipid exchange in apoptosis.

Titolo:	Pro-apoptotic effect of maize lipid transfer protein on mammalian mitochondria
Autori:	CRIMI, Massimo ASTEGNO, Alessandra ZOCCATELLI, Gianni M. Degli Esposti mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2006
Rivista:	ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Abstract:	To assess the effect of lipids and lipid exchange in the pro-apoptotic release of cytochrome c, we investigated the ability of a plant lipid transfer protein (LTP) to initiate the apoptotic cascade at the mitochondrial level. The results show that maize LTP is able to induce cytochrome c release from the intermembrane space of mouse liver mitochondria without significant mitochondrial swelling, similarly to mouse full-length Bid. This effect is influenced by the presence of specific lipids, since addition of lysolipids like lysophosphatidylcholine strongly stimulates the LTP-induced release of cytochrome c while it is inhibited by removal of endogenous free lipids with a complete suppression of the LTP-induced release of cytochrome c. The results are discussed in light of the possible role of lipid exchange in apoptosis.
Handle:	http://hdl.handle.net/11562/306443
Appare nelle tipologie:	01.01 Articolo in Rivista

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