New perspectives in breath – by – breath determination of alveolar gas exchanges in humans.

Alveolar gas transfer over a given breath (i) was determined in ten subjects at rest and during steady-state cycling at 60, 90 or 120 W as the sum of volume of gas transferred at the mouth plus the changes of the alveolar gas stores. This is given by the gas fraction (F-A) change at constant volume plus the volume change (DeltaV(Ai)) at constant fraction i.e. VAl-1(F-Ai-FAi-1)+F-Ai.(Delta VAi), where VAi-1 is the end-expiratory volume at the beginning of the breath. These quantities, except for VAi-1 can be measured on a single-breath (breath-by-breath) basis and VAi-1 equal to the subject's functional residual capacity (FRC, Auchincloss model). Alternatively, the respiratory cycle can be defined as the interval elapsing between two equal expiratory gas fractions in two successive breaths (Gronlund model G). In this case, F-t1=F-t2 and thus the term VAi-1 (F-Ai-FAi-1) vanishes. In the present study, average alveolar O-2 uptake ((V) over dot O-2,O-A) and CO2 output ((V) over dot CO2,A) were equal in both approaches whereby the mean signal-to-noise ratio (S/N) was 40% larger in G. Other approaches yield steady state S/N values equal to that obtained in G, although they are based on the questionable assumption that the inter-breath variability of alveolar gas transfer is minimal. It is concluded that the only promising approach for assessing "true" single breath alveolar gas transfer is that originally proposed by Gronlund.