The level of mRNA for poly(ADP-ribose) polymerase in human PBMC increased 8 h after addition of PHA, reaching its maximum (9-fold over the basal level) 3-4 days after the stimulation and decreasing thereafter. mRNA maximum slightly preceded in time the maximal value of DNA synthesis. The half-life of poly(ADP-ribose) polymerase mRNA, which is 1.2 h in quiescent PBMC, increased up to 3.4 h in stimulated PBMC. This PHA-induced stabilization of the mRNA for poly(ADP-ribose) polymerase could account for the accumulation of the transcript in mitogen-treated PBMC.
Regulation of poly(ADP-ribose) polymerase gene expression in mitogen-stimulated human peripheral blood mononuclear cells
MENEGAZZI, Marta Vittoria;CARCERERI DE PRATI, Alessandra;SUZUKI, Hisanori;LIBONATI, Massimo
1992-01-01
Abstract
The level of mRNA for poly(ADP-ribose) polymerase in human PBMC increased 8 h after addition of PHA, reaching its maximum (9-fold over the basal level) 3-4 days after the stimulation and decreasing thereafter. mRNA maximum slightly preceded in time the maximal value of DNA synthesis. The half-life of poly(ADP-ribose) polymerase mRNA, which is 1.2 h in quiescent PBMC, increased up to 3.4 h in stimulated PBMC. This PHA-induced stabilization of the mRNA for poly(ADP-ribose) polymerase could account for the accumulation of the transcript in mitogen-treated PBMC.
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