Results of gel filtration experiments performed with two different chromatographic media (Superose 12 HR 10/30 and Superdex 75 HR 10/30) and of polyacrylamide gel electrophoresis under non-denaturing and denaturing conditions suggest that aggregated dimers of bovine RNase A, obtained by lyophilization of the enzyme from 40% acetic acid solutions (5 mg RNase A per mi), might consist of two differently structured forms. These two species have slightly different retention times in gel-filtration experiments and migrate differently in electrophoresis under non-denaturing conditions. The fast migrating dimer in non-denaturing gel electrophoresis is able to degrade double-stranded poly(A) poly(U) more efficiently than the other, and the two forms are found in a ratio of about 3:1. (C) 1998 Elsevier Science B,V. All rights reserved.

Two different forms of aggregated dimers of ribonuclease A

GOTTE, Giovanni;LIBONATI, Massimo
1998

Abstract

Results of gel filtration experiments performed with two different chromatographic media (Superose 12 HR 10/30 and Superdex 75 HR 10/30) and of polyacrylamide gel electrophoresis under non-denaturing and denaturing conditions suggest that aggregated dimers of bovine RNase A, obtained by lyophilization of the enzyme from 40% acetic acid solutions (5 mg RNase A per mi), might consist of two differently structured forms. These two species have slightly different retention times in gel-filtration experiments and migrate differently in electrophoresis under non-denaturing conditions. The fast migrating dimer in non-denaturing gel electrophoresis is able to degrade double-stranded poly(A) poly(U) more efficiently than the other, and the two forms are found in a ratio of about 3:1. (C) 1998 Elsevier Science B,V. All rights reserved.
RNase A; RNase A aggregate; RNase A dimer; different RNase A dimer; double-stranded RNA
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/230379
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