In this paper an ultramicromethod for the determination of the serum glutamic-oxalacetic and glutamic-pyruvic transaminase activity is presented; this method is based on the use of glutamate dehydrogenase for the enzymatic estimation of the glutamate formed. The dehydrogenation of the glutamate gives rise to the reduction of a diazonium salt, and it is possible to perform a photometric reading of the colored compound at 520 nm. 20 μl of serum and an incubation time of only 45 min at the temperature of 37° were necessary. The normal values never exceeded 54.5 I.U. for the serum glutamic-oxalacetic transaminase and 52 I.U. for the glutamic-pyruvic transaminase. Under conditions of viral hepatitis values of 390 I.U. for glutamic-pyruvic transaminase and 310 I.U. for serum glutamic-oxalacetic transaminase were obtained. © 1970.
A new colorimetric ultramicromethod for serum glutamicoxalacetic and glutamic-pyruvic transaminase determination
GUIDI, Giancesare
1970-01-01
Abstract
In this paper an ultramicromethod for the determination of the serum glutamic-oxalacetic and glutamic-pyruvic transaminase activity is presented; this method is based on the use of glutamate dehydrogenase for the enzymatic estimation of the glutamate formed. The dehydrogenation of the glutamate gives rise to the reduction of a diazonium salt, and it is possible to perform a photometric reading of the colored compound at 520 nm. 20 μl of serum and an incubation time of only 45 min at the temperature of 37° were necessary. The normal values never exceeded 54.5 I.U. for the serum glutamic-oxalacetic transaminase and 52 I.U. for the glutamic-pyruvic transaminase. Under conditions of viral hepatitis values of 390 I.U. for glutamic-pyruvic transaminase and 310 I.U. for serum glutamic-oxalacetic transaminase were obtained. © 1970.
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