A new micromethod for the determination of serum lipase activity is presented. Small quantities of serum are incubated for 30 min at 40° in a substrate consisting of an olive oil suspension in the presence of deoxycholic acid. The pH is 8.5. The hydrolyzed fatty acids are determined with a photometric technique, with a slight modification of the method of Duncombe. The normal values are lower than 20 I.U. © 1972.

A rapid photometric micromethod for serum lipase determination.

GUIDI, Giancesare
1972-01-01

Abstract

A new micromethod for the determination of serum lipase activity is presented. Small quantities of serum are incubated for 30 min at 40° in a substrate consisting of an olive oil suspension in the presence of deoxycholic acid. The pH is 8.5. The hydrolyzed fatty acids are determined with a photometric technique, with a slight modification of the method of Duncombe. The normal values are lower than 20 I.U. © 1972.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/229844
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