A multimembrane enzyme reactor, operating under an electric field, is described. This reactor is based on the concept of isoelectric traps, by which a pair of membranes, of appropriate pi value, are able to trap the enzyme, the buffering ions (which have to be amphoteric), the substrate and, if needed, cofactors. Charged reaction products can thus leave the enzyme chamber and be recovered in other chambers where they are trapped by the same isoelectric mechanism (if amphoteric). The unique advantages of such reactors are absence of enzyme inhibition by a feed-back mechanism and reaction yields approaching 100 %. Examples of a urease and a trypsin/casein reactor are given.

A multimembrane enzyme reactor operating in an electric field.

BOSSI, Alessandra Maria
1997

Abstract

A multimembrane enzyme reactor, operating under an electric field, is described. This reactor is based on the concept of isoelectric traps, by which a pair of membranes, of appropriate pi value, are able to trap the enzyme, the buffering ions (which have to be amphoteric), the substrate and, if needed, cofactors. Charged reaction products can thus leave the enzyme chamber and be recovered in other chambers where they are trapped by the same isoelectric mechanism (if amphoteric). The unique advantages of such reactors are absence of enzyme inhibition by a feed-back mechanism and reaction yields approaching 100 %. Examples of a urease and a trypsin/casein reactor are given.
immobilized enzyme reactors, isoelectric focusing, immobilized pH gradients, capillary electrophoresis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/226573
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