Shoot regeneration from leaf explants has been achieved in Nipponanthemum nipponicum (syn. Chrysanthemum nipponicum), a diploid-related species to cultivated hexaploid chrysanthemums. Effects of 6-benzylaminopurine (BA) and indolacetic acid (IAA), each at three levels of concentration, were tested on three progenies derived from self-fertilisation of a cultivated clone. Optimal BA concentration for regeneration was 2.2 μM, while no significant differences were detected between the IAA concentrations tested. Significant differences between the progenies were observed. Several genotypes were then tested for their shoot-forming ability. Seven out of 23 did not produce shoots, six produced shoots in more than 50% of the explants. The mean number of shoots per regenerating explant was up to three
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