Background: The Investigator Argus Y-28 QS Kit is a 27-locus, six-dye multiplex, which includes six rapidly mutating Short Tandem Repeats (RM Y-STRs). Methods: A new text file containing the optimized panel and bin sets for GeneMapper ID-X Software v1.6 has been prepared. In addition, an internal validation study was conducted on the Investigator Argus Y-28 QS kit using the SeqStudio™ Genetic Analyzer for HID. Results: The full results of that study are presented here, including data on precision, sensitivity, reproducibility, stutter determination, and reduction volume analysis. A concordance study and three case reports are documented. Finally, the kit was successfully used to conduct a proficiency test. Conclusions: The obtained findings achieved a satisfactory performance of the Investigator Argus Y-28 QS Kit on the SeqStudio™ Genetic Analyzer for HID.

Validation of the investigator argus y-28 qs kit on the seqstudio™ genetic analyzer for hid: a new panel file for genemapper id-x software v1.6

Soldati, Giulia
;
Turrina, Stefania;Ausania, Francesco
2025-01-01

Abstract

Background: The Investigator Argus Y-28 QS Kit is a 27-locus, six-dye multiplex, which includes six rapidly mutating Short Tandem Repeats (RM Y-STRs). Methods: A new text file containing the optimized panel and bin sets for GeneMapper ID-X Software v1.6 has been prepared. In addition, an internal validation study was conducted on the Investigator Argus Y-28 QS kit using the SeqStudio™ Genetic Analyzer for HID. Results: The full results of that study are presented here, including data on precision, sensitivity, reproducibility, stutter determination, and reduction volume analysis. A concordance study and three case reports are documented. Finally, the kit was successfully used to conduct a proficiency test. Conclusions: The obtained findings achieved a satisfactory performance of the Investigator Argus Y-28 QS Kit on the SeqStudio™ Genetic Analyzer for HID.
2025
GeneMapper ID-X Software; Internal validation study; Panels and Bin; SeqStudio™ Genetic Analyzer for HID; Y chromosome Short Tandem Repeats
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1165513
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