In this report we describe a patient with persistent neutropenia whose neutrophils showed increased adhesion in a microplate assay. In three separate assays, from 12.5% to 13.7% of the patient's blood neutrophils exhibited spontaneous (unstimulated) adhesion to fetal bovine serum-coated microplate wells, much higher than adhesion of cells from healthy controls (1.9% +/- 2.5 SD, n = 20). The difference of spontaneous adhesion between the patient's and control neutrophils was even higher when cells from a skin-window exudate were examined (patient: 42.1-100% adhesion; control: 3.6% +/- 3.5 SD, n = 20). Over 80% inhibition of the increased adhesion was produced by the 60.3 anti-CD 18 monoclonal antibody, suggesting an involvement of beta 2-integrins.
Increased in vitro neutrophil adherence in a case of chronic idiopathic neutropenia
BIASI, Domenico;BAMBARA, Lisa Maria;Carletto, Antonio;Caramaschi, Paola;Ortolani, Riccardo;PERONA, Giuseppe;BELLAVITE, Paolo
1994-01-01
Abstract
In this report we describe a patient with persistent neutropenia whose neutrophils showed increased adhesion in a microplate assay. In three separate assays, from 12.5% to 13.7% of the patient's blood neutrophils exhibited spontaneous (unstimulated) adhesion to fetal bovine serum-coated microplate wells, much higher than adhesion of cells from healthy controls (1.9% +/- 2.5 SD, n = 20). The difference of spontaneous adhesion between the patient's and control neutrophils was even higher when cells from a skin-window exudate were examined (patient: 42.1-100% adhesion; control: 3.6% +/- 3.5 SD, n = 20). Over 80% inhibition of the increased adhesion was produced by the 60.3 anti-CD 18 monoclonal antibody, suggesting an involvement of beta 2-integrins.
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