Grafting of recovered shoots reduces bois noir disease incidence in vineyard

Bois noir (BN) disease of grapevine is associated with 'Candidatus Phytoplasma solani' (CaPsol). Due to its multifaceted ecology, BN control is extremely difficult. Several studies showed that BN recovery can be elicited by abiotic stresses and treatment with resistance inducers. In this study, field trials along with molecular analyses have been conducted to evaluate if grafting of recovered shoots can increase the BN recovery rate in symptomatic grapevines and decrease the new CaPsol infection rate on asymptomatic grapevines. Field trials were performed in two vineyards (cv. Chardonnay/Kober 5BB) in Franciacorta (Lombardy Region, northern Italy). Grafting ef-fects were evaluated by symptom observation and CaPsol detection by nested-PCR stamp gene amplification for three consecutive years and compared with non-grafted control vines. Obtained data showed that BN incidence was lower in grafted plants, mainly due to a statistically significant increase of recovery rate, four times higher than in non-grafted plants. These data indicated that grafting of recovered shoots can efficiently induce BN recovery, opening an interesting scenario for its utilization in sustainable strategies of vineyard management.