Although several pre-analytical (i.e., specimen collection, preservation, storage, transportation, preparation, inactivation and RNA extraction), analytical (e.g., analytical sensitivity and interfering substances, usage of different gene targets and diverse amplification and detection techniques) and postanalytical (e.g., calibration, test results interpretation, correlation with viral load) aspects may bias the importance of reporting the cycle threshold (Ct) values in SARS-CoV-2 positive samples, the clinical advantages of using accurate and standardized molecular assays fit for this purpose may ultimately offset the limitations. These include the facts that the SARS-CoV-2 Ct predicts emergence and progression of SARS-CoV-2 outbreaks, the risk of developing severe/critical forms of COVID-19 illness and infectiousness, even in in patients with vaccine breakthrough.
The many clinical advantages of reporting the cycle threshold (Ct) value
Lippi, Giuseppe
;
2022-01-01
Abstract
Although several pre-analytical (i.e., specimen collection, preservation, storage, transportation, preparation, inactivation and RNA extraction), analytical (e.g., analytical sensitivity and interfering substances, usage of different gene targets and diverse amplification and detection techniques) and postanalytical (e.g., calibration, test results interpretation, correlation with viral load) aspects may bias the importance of reporting the cycle threshold (Ct) values in SARS-CoV-2 positive samples, the clinical advantages of using accurate and standardized molecular assays fit for this purpose may ultimately offset the limitations. These include the facts that the SARS-CoV-2 Ct predicts emergence and progression of SARS-CoV-2 outbreaks, the risk of developing severe/critical forms of COVID-19 illness and infectiousness, even in in patients with vaccine breakthrough.
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