: Intra-articular injection of mesenchymal stem cells has shown benefit for the treatment of osteoarthritis (OA). However, mesenchymal stem/stromal cells at the origin of these clinical results are heterogenous cell populations with limited cellular characterization. Here, two transgenic reporter mice were used to examine the differential effects of two precisely defined perivascular cell populations (Pdgfrα+  and Pdgfrβ+  cells) from white adipose tissue for alleviation of OA. Perivascular mesenchymal cells were isolated from transgenic Pdgfrα-and Pdgfrβ-CreERT2 reporter animals and delivered as a one-time intra-articular dose to C57BL/6J mice after destabilization of the medial meniscus (DMM). Both Pdgfrα+  and Pdgfrβ+  cell preparations improved metrics of cartilage degradation and reduced markers of chondrocyte hypertrophy. While some similarities in cell distribution were identified within the synovial and perivascular spaces, injected Pdgfrα+  cells remained in the superficial layers of articular cartilage, while Pdgfrβ+  cells were more widely dispersed. Pdgfrβ+  cell therapy prevented subchondral sclerosis induced by DMM, while Pdgfrα+  cell therapy had no effect. In summary, while both cell therapies showed beneficial effects in the DMM model, important differences in cell incorporation, persistence, and subchondral sclerosis were identified.

Divergent effects of distinct perivascular cell subsets for intra-articular cell therapy in posttraumatic osteoarthritis

Negri, Stefano;
2021-01-01

Abstract

: Intra-articular injection of mesenchymal stem cells has shown benefit for the treatment of osteoarthritis (OA). However, mesenchymal stem/stromal cells at the origin of these clinical results are heterogenous cell populations with limited cellular characterization. Here, two transgenic reporter mice were used to examine the differential effects of two precisely defined perivascular cell populations (Pdgfrα+  and Pdgfrβ+  cells) from white adipose tissue for alleviation of OA. Perivascular mesenchymal cells were isolated from transgenic Pdgfrα-and Pdgfrβ-CreERT2 reporter animals and delivered as a one-time intra-articular dose to C57BL/6J mice after destabilization of the medial meniscus (DMM). Both Pdgfrα+  and Pdgfrβ+  cell preparations improved metrics of cartilage degradation and reduced markers of chondrocyte hypertrophy. While some similarities in cell distribution were identified within the synovial and perivascular spaces, injected Pdgfrα+  cells remained in the superficial layers of articular cartilage, while Pdgfrβ+  cells were more widely dispersed. Pdgfrβ+  cell therapy prevented subchondral sclerosis induced by DMM, while Pdgfrα+  cell therapy had no effect. In summary, while both cell therapies showed beneficial effects in the DMM model, important differences in cell incorporation, persistence, and subchondral sclerosis were identified.
DMM
Pdgfrα
Pdgfrβ
adventitial cell
cell therapy
osteoarthritis
synovium
Animals
Cell- and Tissue-Based Therapy
Disease Models, Animal
Injections, Intra-Articular
Mice
Mice, Inbred C57BL
Mice, Transgenic
Receptor, Platelet-Derived Growth Factor alpha
Sclerosis
Cartilage, Articular
Osteoarthritis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1062222
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