Bombyx mori silk fibroin (SF) is a biomacromolecule allowing to assemble scaffolds 7 for tissue engineering/regeneration purposes because of its cellular adhesiveness, high 8 biocompatibility, and low immunogenicity. Earlier work showed that two types of 3D-SF-based 9 nonwovens (3D-SFnws) implanted into mouse subcutaneous tissue were promptly vascularised via plates in exosome-depleted medium. DNA amounts and D-glucose consumption revealed HDFs 17 undefined molecular mechanisms. This study used nontumorigenic adult human dermal fibroblasts (HDFs) adhering to a third type of 3D-SFnws to test whether HDFs release exosomes whose contents promote neoangiogenesis.

Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis

Hu Peng;Chiarini Anna
;
Wu Jun;Armato Ubaldo;Dal Prà Ilaria
Supervision
2021-01-01

Abstract

Bombyx mori silk fibroin (SF) is a biomacromolecule allowing to assemble scaffolds 7 for tissue engineering/regeneration purposes because of its cellular adhesiveness, high 8 biocompatibility, and low immunogenicity. Earlier work showed that two types of 3D-SF-based 9 nonwovens (3D-SFnws) implanted into mouse subcutaneous tissue were promptly vascularised via plates in exosome-depleted medium. DNA amounts and D-glucose consumption revealed HDFs 17 undefined molecular mechanisms. This study used nontumorigenic adult human dermal fibroblasts (HDFs) adhering to a third type of 3D-SFnws to test whether HDFs release exosomes whose contents promote neoangiogenesis.
2021
SilK fibroin, nonwovens, dermis, human fibroblast, human endothelial cell, exosome, cytokine, chemokine, angiogenesis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1036330
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