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EnglishPoint mutations in the Kirsten rat sarcoma viral oncogene homologue (KRAS) gene are being increasingly recognized as Important diagnostic and prognostic markers in cancer. In this work, we describe a rapid and low-cost method for the naked-eye detection of cancer-related point mutations In KRAS based on gold nanoparticles. This simple colorimetric assay is sensitive (limit of detection in the low picomolar range), Instrument-free, and employs nonstringent room temperature conditions due to a combination of DNA-conjugated gold nanoparticles, a probe design which exploits cooperative hybridization for increased binding affinity, and signal enhancement on the surface of magnetic beads. Additionally, the scheme Is suitable for point-of-care applications, as it combines naked-eye detection, small sample volumes, and isothermal (PCR-free) amplification.
| Titolo: | Gold-Nanoparticle-Based Colorimetric Discrimination of Cancer-Related Point Mutations with Picomolar Sensitivity |
| Autori: | |
| Data di pubblicazione: | 2013 |
| Rivista: | |
| Handle: | http://hdl.handle.net/11562/1025855 |
| Appare nelle tipologie: | 01.01 Articolo in Rivista |
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http://hdl.handle.net/11562/1025855
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