The coordination features and signaling of a L-lactate ion by a [Tb(bpcd)](+) (bpcd = N,N'-bis(2-pyridylmethyl)-trans-1,2-diaminocyclohexane-N,N'-diacetate) complex have been investigated by means of a combination of techniques, including total luminescence, calorimetry and circularly polarized luminescence. The L-lactate/[Tb(bpcd)](+) association constant, determined by both luminescence titration and isothermal titration calorimetry, indicates a weak interaction (log K = 1.3-1.45) between the analyte and both enantiomers of the complex. The theoretical DFT calculations suggest that the most likely coordination of L-lactate to the possible stereoisomers of the [Tb(S,S-bpcd)](+) complex (trans-O,O or trans-N-py, N-py) is one involving a hydroxyl group. The results of [Tb(rac-bpcd)](+) as a chiroptical luminescent probe of L-lactate underline the peculiar role of the chiral 1,2-diaminocyclohexane (DACH) backbone. Indeed, the target anion is capable of inducing CPL activity in the racemic mixture of Tb complexes containing DACH-based ligands. The same is not observed for the achiral analogue [Tb(bped)](+) (bped = N, N'-bis(2-pyridylmethyl) ethylenediamine-N,N'-diacetate) complex, likely because of the flexibility of the ethylenic group which allows an interconversion between different isomers which produces a null net CPL activity. Thanks to the differential quantum yield of the two diastereomeric species (R,R)-L and (S,S)-L, one can use the racemic complex to reveal L-lactate by measuring the induced CPL spectrum. Interestingly, this has been demonstrated in a commercial complex solution for medical use, containing several electrolytes, namely Ringer's lactate.

A chiral lactate reporter based on total and circularly polarized Tb(iii) luminescence

Leonzio, Marco;Bettinelli, Marco;Piccinelli, Fabio
2018-01-01

Abstract

The coordination features and signaling of a L-lactate ion by a [Tb(bpcd)](+) (bpcd = N,N'-bis(2-pyridylmethyl)-trans-1,2-diaminocyclohexane-N,N'-diacetate) complex have been investigated by means of a combination of techniques, including total luminescence, calorimetry and circularly polarized luminescence. The L-lactate/[Tb(bpcd)](+) association constant, determined by both luminescence titration and isothermal titration calorimetry, indicates a weak interaction (log K = 1.3-1.45) between the analyte and both enantiomers of the complex. The theoretical DFT calculations suggest that the most likely coordination of L-lactate to the possible stereoisomers of the [Tb(S,S-bpcd)](+) complex (trans-O,O or trans-N-py, N-py) is one involving a hydroxyl group. The results of [Tb(rac-bpcd)](+) as a chiroptical luminescent probe of L-lactate underline the peculiar role of the chiral 1,2-diaminocyclohexane (DACH) backbone. Indeed, the target anion is capable of inducing CPL activity in the racemic mixture of Tb complexes containing DACH-based ligands. The same is not observed for the achiral analogue [Tb(bped)](+) (bped = N, N'-bis(2-pyridylmethyl) ethylenediamine-N,N'-diacetate) complex, likely because of the flexibility of the ethylenic group which allows an interconversion between different isomers which produces a null net CPL activity. Thanks to the differential quantum yield of the two diastereomeric species (R,R)-L and (S,S)-L, one can use the racemic complex to reveal L-lactate by measuring the induced CPL spectrum. Interestingly, this has been demonstrated in a commercial complex solution for medical use, containing several electrolytes, namely Ringer's lactate.
2018
COMPLEX-FORMATION; EUROPIUM(III) COMPLEXES; WATER-MOLECULES; SPECTROSCOPY; EMISSION; ACID; SPECIATION; STABILITY; CONSTANTS; LIGANDS
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1017236
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