Summary Objective: To evaluate HIV-1 DNA load in PBLs and monocytes from both long-term HAART-treated and antiretroviral naı¨ve HIV-1 infected patients. Methods: Cross-sectional quantitative analysis of HIV-1 DNA load was performed in PBLs and monocytes, purified from 34 long-term HAART-treated and 34 naı¨ve HIV-1 infected patients, and compared to RNA viral load and CD4þ cell count. Results: HAART-treated patients showed significantly lower levels of viral DNA both in PBLs and monocytes in comparison with naı¨ve individuals. Variable levels of HIV-1 DNA amount in monocytes were detected in all naı¨ve patients but only in 12 of 34 HAART-treated individuals. PBLs HIV-1 DNA load was inversely correlated to CD4þ cell count in naı¨ve and HAART-treated patients whereas no association was detected in monocytes. Conclusions: Long-term HAART decreased HIV-1 DNA load in PBLs and monocytes demonstrating a valuable inhibitor effect, especially in short-lived reservoirs. In addition, the positive correlation of DNA burden between PBLs and monocytes may suggest a dynamic relation between these reservoirs in the course of disease. HIV-1 DNA load quantitative analysis in PBLs and monocytes may be considered an important approach to study the HIV-1 reservoir and the effectiveness of HAART therapy in HIV-1 seropositive patients.
HIV-1 DNA load analysis in peripheral blood lymphocytes and monocytes from naïve and HAART-treated individuals.
Gibellini D
;
2008-01-01
Abstract
Summary Objective: To evaluate HIV-1 DNA load in PBLs and monocytes from both long-term HAART-treated and antiretroviral naı¨ve HIV-1 infected patients. Methods: Cross-sectional quantitative analysis of HIV-1 DNA load was performed in PBLs and monocytes, purified from 34 long-term HAART-treated and 34 naı¨ve HIV-1 infected patients, and compared to RNA viral load and CD4þ cell count. Results: HAART-treated patients showed significantly lower levels of viral DNA both in PBLs and monocytes in comparison with naı¨ve individuals. Variable levels of HIV-1 DNA amount in monocytes were detected in all naı¨ve patients but only in 12 of 34 HAART-treated individuals. PBLs HIV-1 DNA load was inversely correlated to CD4þ cell count in naı¨ve and HAART-treated patients whereas no association was detected in monocytes. Conclusions: Long-term HAART decreased HIV-1 DNA load in PBLs and monocytes demonstrating a valuable inhibitor effect, especially in short-lived reservoirs. In addition, the positive correlation of DNA burden between PBLs and monocytes may suggest a dynamic relation between these reservoirs in the course of disease. HIV-1 DNA load quantitative analysis in PBLs and monocytes may be considered an important approach to study the HIV-1 reservoir and the effectiveness of HAART therapy in HIV-1 seropositive patients.
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