We present an NMR method based on natural abundance O-17 relaxation of water to determine effective viscosity in biological aqueous samples. The method accurately captures viscosity of dilute and crowded protein solutions and offers a fairly simple way to quantify the internal fluidity of biological condensates formed through phase separation.

A facile oxygen-17 NMR method to determine effective viscosity in dilute, molecularly crowded and confined aqueous media

Munari, Francesca;Assfalg, Michael;
2019-01-01

Abstract

We present an NMR method based on natural abundance O-17 relaxation of water to determine effective viscosity in biological aqueous samples. The method accurately captures viscosity of dilute and crowded protein solutions and offers a fairly simple way to quantify the internal fluidity of biological condensates formed through phase separation.
2019
NMR spectroscopy, viscosity, molecular crowding
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1012611
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