Next-generation sequencing technologies have allowed new tools to be developed for transcriptomecharacterization.We describe here the methods for the preparation of a library from pooled RNAs for 454 sequencingof 3¢-cDNA fragments. We also describe how the read sequences obtained can be used to generate,through de novo reads assembly, a large catalog of unique transcripts in organisms for which a comprehensivecollection of transcripts or the complete genome sequence is not available. Finally, this catalogcan be used efficiently to design oligonucleotide probes for setting up a comprehensive microarray forglobal analysis of gene expression.

Generation of a large catalog of unique transcripts for whole-genome expression analysis in non-model species

BELLIN, Diana;FERRARINI, Alberto;DELLEDONNE, Massimo
2011-01-01

Abstract

Next-generation sequencing technologies have allowed new tools to be developed for transcriptomecharacterization.We describe here the methods for the preparation of a library from pooled RNAs for 454 sequencingof 3¢-cDNA fragments. We also describe how the read sequences obtained can be used to generate,through de novo reads assembly, a large catalog of unique transcripts in organisms for which a comprehensivecollection of transcripts or the complete genome sequence is not available. Finally, this catalogcan be used efficiently to design oligonucleotide probes for setting up a comprehensive microarray forglobal analysis of gene expression.
2011
978-1-61779-065-2
genomica; trascrittomica; next generation sequencing
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/346970
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