Investigating at transmission electron microscopy the intracellular trafficking of hyaluronic acid-based nanoparticles remains a challenge due to their intrinsic weak electron density. Here we describe a simple protocol to stain hyaluronic acid that allows visualization of hyaluronic acid-based nanoparticles inside cells at both light and electron microscopy. By applying the critical-electrolyte-concentration Alcian blue method, these nanoparticles were observed as blue dots at bright-field microscopy or filled with fine electron dense precipitates at transmission electron microscopy.

Alcian blue staining to visualize intracellular hyaluronic acid-based nanoparticles

Repellin, Mathieu
;
Carton, Flavia;Malatesta, Manuela
2023-01-01

Abstract

Investigating at transmission electron microscopy the intracellular trafficking of hyaluronic acid-based nanoparticles remains a challenge due to their intrinsic weak electron density. Here we describe a simple protocol to stain hyaluronic acid that allows visualization of hyaluronic acid-based nanoparticles inside cells at both light and electron microscopy. By applying the critical-electrolyte-concentration Alcian blue method, these nanoparticles were observed as blue dots at bright-field microscopy or filled with fine electron dense precipitates at transmission electron microscopy.
978-1-0716-2675-7
Histochemistry
Hyaluronate
Light microscopy
Nanocarriers
Transmission electron microscopy
Alcian Blue
Coloring Agents
Staining and Labeling
Hyaluronic Acid
Nanoparticles
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1079707
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