Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population composed of mature and immature cells of myeloid origin that play a major role in tumor progression by inhibiting the antitumor immune responses mediated by T cells. In this chapter, we describe protocols for isolation, phenotypical and functional evaluation of MDSCs isolated from mouse tumors, with the aim at unifying and standardizing protocols set up by different laboratories.

Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells

Barouni RM
Membro del Collaboration Group
;
Musiu C
Membro del Collaboration Group
;
Bronte V
Membro del Collaboration Group
;
Ugel S
Membro del Collaboration Group
;
Canè S.
Membro del Collaboration Group
2021

Abstract

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population composed of mature and immature cells of myeloid origin that play a major role in tumor progression by inhibiting the antitumor immune responses mediated by T cells. In this chapter, we describe protocols for isolation, phenotypical and functional evaluation of MDSCs isolated from mouse tumors, with the aim at unifying and standardizing protocols set up by different laboratories.
Myeloid-derived suppressor cells (MDSCs), Tumor digestion, Immunosuppression, ELISPOT, Cell sorting
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1060782
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