Platelets are nowadays more and more investigated to better characterise their role in many different pathophysiological processes. The present thesis summarises explore NAFLD (Non-Alcoholic Fatty Liver Disease) is a chronic epatopathy degenerating in NASH syndrome and in the worst scenario to liver cancer. Using a Cellix Microfluidic platform with collagen coated microchip and a Flow Cytometry (FACS) on Platelet derived Microvesicles (PMVs) essay, a stronger platelet activity and higher release of PMVs in such patients, is highlighted, probably due to a preactivation platelet status. A platelet activation, leading to wider platelets aggregates, is observed after incubation with C6O4 (100-200 ng/mL), a new compound belonging to Perfluoroalkyl substances (PFAS), used in detergent, coatings for cookware etc. Aspirin 100µM (ASA) shows a neutralisation of C6O4 procoagulant properties. Similar results are observed in FACS analysis on PMVs, regardless the co-stimulation with ADP 7.5µM, TRAP 10µM or in resting condi

PLATELETS BEYOND ATHEROTHROMBOSIS

Castelli Marco
2021

Abstract

Platelets are nowadays more and more investigated to better characterise their role in many different pathophysiological processes. The present thesis summarises explore NAFLD (Non-Alcoholic Fatty Liver Disease) is a chronic epatopathy degenerating in NASH syndrome and in the worst scenario to liver cancer. Using a Cellix Microfluidic platform with collagen coated microchip and a Flow Cytometry (FACS) on Platelet derived Microvesicles (PMVs) essay, a stronger platelet activity and higher release of PMVs in such patients, is highlighted, probably due to a preactivation platelet status. A platelet activation, leading to wider platelets aggregates, is observed after incubation with C6O4 (100-200 ng/mL), a new compound belonging to Perfluoroalkyl substances (PFAS), used in detergent, coatings for cookware etc. Aspirin 100µM (ASA) shows a neutralisation of C6O4 procoagulant properties. Similar results are observed in FACS analysis on PMVs, regardless the co-stimulation with ADP 7.5µM, TRAP 10µM or in resting condi
Platelet, NAFLD, Kca1.1, PFAS
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1049287
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