PLATELETS BEYOND ATHEROTHROMBOSIS

Platelets are nowadays more and more investigated to better characterise their role in many different pathophysiological processes. The present thesis summarises explore NAFLD (Non-Alcoholic Fatty Liver Disease) is a chronic epatopathy degenerating in NASH syndrome and in the worst scenario to liver cancer. Using a Cellix Microfluidic platform with collagen coated microchip and a Flow Cytometry (FACS) on Platelet derived Microvesicles (PMVs) essay, a stronger platelet activity and higher release of PMVs in such patients, is highlighted, probably due to a preactivation platelet status. A platelet activation, leading to wider platelets aggregates, is observed after incubation with C6O4 (100-200 ng/mL), a new compound belonging to Perfluoroalkyl substances (PFAS), used in detergent, coatings for cookware etc. Aspirin 100µM (ASA) shows a neutralisation of C6O4 procoagulant properties. Similar results are observed in FACS analysis on PMVs, regardless the co-stimulation with ADP 7.5µM, TRAP 10µM or in resting condi