Targeting cancer cells with antibodies is a powerful methodology for both tumor imaging and drug delivery. We show how surface enhanced resonance Raman scattering (SERRS) signals of single cells can be obtained engineering gold nanoparticles synthesized by laser ablation. In particular we will use an antibody (D2/B) recognizing the prostate specific membrane antigen (PSMA), overexpressed both in prostate tumors in patients and on LNCaP prostate cancer cells (our “in vitro” tumor model), and Texas Red as SERRS reporter. High intensity signals are observed for LNCaP cells incubated with the targeted nanoparticles and comparison with DU145 cells, which lack PSMA, shows the high targeting specificity of the prepared nanoparticles.
SERS IMAGING OF CANCER CELLS WITH ANTIBODY FUNCTIONALIZED GOLD NANOPARTICLES
BOSCAINI, Anita;FRACASSO, Giulio;COLOMBATTI, Marco
2010-01-01
Abstract
Targeting cancer cells with antibodies is a powerful methodology for both tumor imaging and drug delivery. We show how surface enhanced resonance Raman scattering (SERRS) signals of single cells can be obtained engineering gold nanoparticles synthesized by laser ablation. In particular we will use an antibody (D2/B) recognizing the prostate specific membrane antigen (PSMA), overexpressed both in prostate tumors in patients and on LNCaP prostate cancer cells (our “in vitro” tumor model), and Texas Red as SERRS reporter. High intensity signals are observed for LNCaP cells incubated with the targeted nanoparticles and comparison with DU145 cells, which lack PSMA, shows the high targeting specificity of the prepared nanoparticles.
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