The aim of the research is to develop efficient regeneration and genetic transformation protocols to be applied to different varieties of table and wine grapes and to varieties and rootstock of peach. The genetic transformation has been carried out by optimizing the method already developed for two table grape cultivars (Thompson seedless and Silcora) by Mezzetti et al., 2002. This method relays on in vitro production of meristematic bulk tissues with a strong capacity to differentiate adventitious shoots via organogenesis. Slices prepared from these bulks were used for Agrobacterium-mediated transformation of table and wine grape varieties (Vitroblack and Pinot Noir) and a commonly used peach variety (Big Top) and rootstock (GF677).
Regeneration and genetic transformation via-organogenesis of different varieties of Vitis vinifera and Prunus persica
PANDOLFINI, Tiziana;
2011-01-01
Abstract
The aim of the research is to develop efficient regeneration and genetic transformation protocols to be applied to different varieties of table and wine grapes and to varieties and rootstock of peach. The genetic transformation has been carried out by optimizing the method already developed for two table grape cultivars (Thompson seedless and Silcora) by Mezzetti et al., 2002. This method relays on in vitro production of meristematic bulk tissues with a strong capacity to differentiate adventitious shoots via organogenesis. Slices prepared from these bulks were used for Agrobacterium-mediated transformation of table and wine grape varieties (Vitroblack and Pinot Noir) and a commonly used peach variety (Big Top) and rootstock (GF677).
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