The early nodulin N5 of Medicago truncatula is a lipid transfer protein that has been proven to positively regulate nodulation, although it displays in vitro inhibitory activity against Sinorhizobium meliloti. We utilized a RNAi-based hairpin construct to down-regulate MtN5 expression in M. truncatula hairy roots. MtN5-silenced roots inoculated with rhizobia displayed an increased number of root hair curling events and a reduced number of invaded primordia and mature nodules as compared to wild type roots. Nodule primordia formation appeared unaltered in MtN5-silenced roots. This phenotype was associated with the stimulation of ENOD11 expression, an early marker of infection, and with the down-regulation of Flotillin 4(FLOT4), a protein involved in rhizobia entry pathway.
Involvement of Medicago truncatula lipid transfer protein N5 in epidermal phases of rhizobia-host interaction.
PII, Youry;MOLESINI, Barbara;PANDOLFINI, Tiziana
2012-01-01
Abstract
The early nodulin N5 of Medicago truncatula is a lipid transfer protein that has been proven to positively regulate nodulation, although it displays in vitro inhibitory activity against Sinorhizobium meliloti. We utilized a RNAi-based hairpin construct to down-regulate MtN5 expression in M. truncatula hairy roots. MtN5-silenced roots inoculated with rhizobia displayed an increased number of root hair curling events and a reduced number of invaded primordia and mature nodules as compared to wild type roots. Nodule primordia formation appeared unaltered in MtN5-silenced roots. This phenotype was associated with the stimulation of ENOD11 expression, an early marker of infection, and with the down-regulation of Flotillin 4(FLOT4), a protein involved in rhizobia entry pathway.
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