Oenococcus oeni is a lactic acid bacterium of great interest for winemaking, as strains of this species are the most important actors of malolactic fermentation (MLF). MLF, after alcoholic fermentation performed by yeasts, is required in certain styles of wine as it could confer positive sensory traits and improves their microbiological stability. In this study a whole transcriptome analysis was performed on the O. oeni model strain PSU-1 by using a Combimatrix chip with probes specific for almost all the ORFs and pseudogenes annotated (http://ddlab.sci.univr.it/fgc/index.php?option=com_content&task=view& id=48). Chosen shock conditions modelled some wine characteristics, such as presence of ethanol (10% v/v), low pH (3.5), and both conditions applied simultaneously; also a heat shock at 42°C was performed. They were applied to cultures in mid-exponential phase for half of the replication time in the medium used. Results showed that the major modifications of gene expression were obtained when heat shock and the combination of pH and ethanol were applied. Only 28 genes are differentially expressed in all shock conditions, including a transcriptional regulator, therefore it is possible to hypothesize that few genes are crucial for the stress response machinery of the cell. The results of the present study have an impact on the comprehension of the general mechanism of response to stresses in O. oeni which are useful not only for oenological applications, but also for a general advancement of the knowledge of low GC Gram positive bacteria. Oenococcus oeni shows several genomic peculiarities, e.g. small genome size and lack of the mismatch repair system, which make it a very interesting bacterium which can be considered a model organism besides Bacillus subtilis and Lactococcus lactis.

The transcriptomic changes of Oenococcus oeni PSU-1 after shock exposure

STEFANELLI, ELENA;FELIS, Giovanna;ZENONI, Sara;FERRARINI, Alberto;FRACCHETTI, Fabio;TONONI, Paola;DELLEDONNE, Massimo;PEZZOTTI, Mario;TORRIANI, Sandra
2011-01-01

Abstract

Oenococcus oeni is a lactic acid bacterium of great interest for winemaking, as strains of this species are the most important actors of malolactic fermentation (MLF). MLF, after alcoholic fermentation performed by yeasts, is required in certain styles of wine as it could confer positive sensory traits and improves their microbiological stability. In this study a whole transcriptome analysis was performed on the O. oeni model strain PSU-1 by using a Combimatrix chip with probes specific for almost all the ORFs and pseudogenes annotated (http://ddlab.sci.univr.it/fgc/index.php?option=com_content&task=view& id=48). Chosen shock conditions modelled some wine characteristics, such as presence of ethanol (10% v/v), low pH (3.5), and both conditions applied simultaneously; also a heat shock at 42°C was performed. They were applied to cultures in mid-exponential phase for half of the replication time in the medium used. Results showed that the major modifications of gene expression were obtained when heat shock and the combination of pH and ethanol were applied. Only 28 genes are differentially expressed in all shock conditions, including a transcriptional regulator, therefore it is possible to hypothesize that few genes are crucial for the stress response machinery of the cell. The results of the present study have an impact on the comprehension of the general mechanism of response to stresses in O. oeni which are useful not only for oenological applications, but also for a general advancement of the knowledge of low GC Gram positive bacteria. Oenococcus oeni shows several genomic peculiarities, e.g. small genome size and lack of the mismatch repair system, which make it a very interesting bacterium which can be considered a model organism besides Bacillus subtilis and Lactococcus lactis.
2011
Oenococcus oeni; transcriptome; stress response
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/653772
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