The present study firstly investigated the composition of both bacterial and fungal communities selected in two sampling points within a forest area (North of Italy) that has been affected by wildfire. Several bacterial and fungal strains were obtained in pure cultures and taxonomical characterized. Furthermore, each strain was tested for its capability of degrading HMW hydrocarbons through plate assay with aromatic dyes in order to establish their potential bioremediation activity. Afterwards, an 'in field' experiment was carried out by comparing two different in-situ bioremediation protocols by means of two commercial products: (i) biostimulation with a fertilizer compound and (ii) bioaugmentation with Trichoderma sp. EV strain. The chemical analyses showed that at the beginning of the experimental trial, the average concentration of C12-40 hydrocarbons was about 180 mg kg-1. This value exceeds the limit establish by the Italian law fixed at 50 mg Kg-1 (D. Lgs. 152/06). Eventually, the biostimulation resulted the most efficient protocol. In fact, the lowest levels - ≤ 50 mg HMW hydrocarbons kg-1 - were reached in 60 days. Interestingly, the inoculation of Trichoderma sp. EV also enhanced the C12-40 degradation in comparison to the natural attenuation (control), which conversely reached the lowest level after 270 days.
Effects of wildfire on the level of high molecular weight (HMW) hydrocarbon compounds in forest soil: analysis of HMW hydrocarbons-degrading microbial communities and comparison of different in-situ bioremediation protocols
ANDREOLLI, Marco;LAMPIS, Silvia;VALLINI, Giovanni
2013-01-01
Abstract
The present study firstly investigated the composition of both bacterial and fungal communities selected in two sampling points within a forest area (North of Italy) that has been affected by wildfire. Several bacterial and fungal strains were obtained in pure cultures and taxonomical characterized. Furthermore, each strain was tested for its capability of degrading HMW hydrocarbons through plate assay with aromatic dyes in order to establish their potential bioremediation activity. Afterwards, an 'in field' experiment was carried out by comparing two different in-situ bioremediation protocols by means of two commercial products: (i) biostimulation with a fertilizer compound and (ii) bioaugmentation with Trichoderma sp. EV strain. The chemical analyses showed that at the beginning of the experimental trial, the average concentration of C12-40 hydrocarbons was about 180 mg kg-1. This value exceeds the limit establish by the Italian law fixed at 50 mg Kg-1 (D. Lgs. 152/06). Eventually, the biostimulation resulted the most efficient protocol. In fact, the lowest levels - ≤ 50 mg HMW hydrocarbons kg-1 - were reached in 60 days. Interestingly, the inoculation of Trichoderma sp. EV also enhanced the C12-40 degradation in comparison to the natural attenuation (control), which conversely reached the lowest level after 270 days.
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