Over the last fifteen years, multiplex PCR typing of Y-chromosomal short tandem repeat (STR) loci has emerged as a powerful tool for forensic casework analysis, especially in sexual assault cases, where evidence typically consists of mixtures containing large amounts of female DNA in the presence of minor amounts of male DNA. However, since Y-STR loci are from nonrecombining portion of the Y chromosome, individuals from the same lineage typically share the same profile. Therefore, Y-STR profiles do not carry the same discrimination power as autosomal profiles. The availability of new multiplex PCR assays that allow simultaneous typing of conventional and additional Y-STRs improves the power of discrimination of paternal lineages and is therefore highly welcomed by the forensic community. Recently Promega launched the PowerPlex® Y23 System, which includes the Y-STR loci found in the AmpFlSTR® Yfiler® PCR Amplification Kit from Life Technologies plus six new loci that display high genetic diversity in human populations. Two of the loci, DYS570 and DYS576, are rapidly mutating loci (1) . The PowerPlex® Y23 System Technical Manual TMD035 describes instrument setup and sample preparation of PCR products using the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Here, we report the successful analysis of amplicons obtained with PowerPlex® Y23 on the ABI PRISM® 310 Genetic Analyzer. We performed sensitivity and mixture studies using control DNA samples and compared typing results in a limited set of casework samples previously typed with the Yfiler® kit.
The Use of PowerPlex® Y23 System with the ABI PRISM® 310 Genetic Analyzer
Caratti, Stefano;
2012-01-01
Abstract
Over the last fifteen years, multiplex PCR typing of Y-chromosomal short tandem repeat (STR) loci has emerged as a powerful tool for forensic casework analysis, especially in sexual assault cases, where evidence typically consists of mixtures containing large amounts of female DNA in the presence of minor amounts of male DNA. However, since Y-STR loci are from nonrecombining portion of the Y chromosome, individuals from the same lineage typically share the same profile. Therefore, Y-STR profiles do not carry the same discrimination power as autosomal profiles. The availability of new multiplex PCR assays that allow simultaneous typing of conventional and additional Y-STRs improves the power of discrimination of paternal lineages and is therefore highly welcomed by the forensic community. Recently Promega launched the PowerPlex® Y23 System, which includes the Y-STR loci found in the AmpFlSTR® Yfiler® PCR Amplification Kit from Life Technologies plus six new loci that display high genetic diversity in human populations. Two of the loci, DYS570 and DYS576, are rapidly mutating loci (1) . The PowerPlex® Y23 System Technical Manual TMD035 describes instrument setup and sample preparation of PCR products using the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Here, we report the successful analysis of amplicons obtained with PowerPlex® Y23 on the ABI PRISM® 310 Genetic Analyzer. We performed sensitivity and mixture studies using control DNA samples and compared typing results in a limited set of casework samples previously typed with the Yfiler® kit.
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