The parallel deposition of polymers made with several functional monomers in a microchip format or onto an appropriate solid support could find a wide range of applications in sensing, combinatorial and high-throughput screening. We here report an innovative fabrication approach for the elaboration of polymer microarrays bearing polymer dots typically 300μm in diameter, fabricated in situ on a glass cover slip via CO2 laser pulse initiated polymerisation. Using such laser in a format that allows spatial positioning or steering of the beam together with appropriate parameters, it is possible to locally heat a surface to the appropriate temperature which initiates thermal polymerisation of suitable monomer mixtures, resulting in spatial patterning of the polymers. Following this approach we report the fabrication of a heterogeneous microarray of 14 polymers on a single microfluidic chip, starting from different commonly used functional monomers. As a different technology to generate polymeric arrays, a parallel pin deposition onto glass slides using a model eight pins machinery system is also presented here. Pins uptakes of 0.8μL functional monomer solutions were followed by dot depositions performed onto dichlorodimethylsilane modified borosilicate glass slides. Photo-polymerisation under a UV lamp for 5min was further performed to obtain and array of millimetre sized polymer dots. We believe that these new technologies may find wide applicability in the fields of combinatorial and high-throughput screening for the development of affinity materials and molecularly imprinted polymers (MIPs), (bio)chemical sensing and material biocompatibility studies, particularly when low sample volumes are available.

FABRICATION OF POLYMER ARRAYS FOR SENSOR TECHNOLOGY

BOSSI, Alessandra Maria;
2008-01-01

Abstract

The parallel deposition of polymers made with several functional monomers in a microchip format or onto an appropriate solid support could find a wide range of applications in sensing, combinatorial and high-throughput screening. We here report an innovative fabrication approach for the elaboration of polymer microarrays bearing polymer dots typically 300μm in diameter, fabricated in situ on a glass cover slip via CO2 laser pulse initiated polymerisation. Using such laser in a format that allows spatial positioning or steering of the beam together with appropriate parameters, it is possible to locally heat a surface to the appropriate temperature which initiates thermal polymerisation of suitable monomer mixtures, resulting in spatial patterning of the polymers. Following this approach we report the fabrication of a heterogeneous microarray of 14 polymers on a single microfluidic chip, starting from different commonly used functional monomers. As a different technology to generate polymeric arrays, a parallel pin deposition onto glass slides using a model eight pins machinery system is also presented here. Pins uptakes of 0.8μL functional monomer solutions were followed by dot depositions performed onto dichlorodimethylsilane modified borosilicate glass slides. Photo-polymerisation under a UV lamp for 5min was further performed to obtain and array of millimetre sized polymer dots. We believe that these new technologies may find wide applicability in the fields of combinatorial and high-throughput screening for the development of affinity materials and molecularly imprinted polymers (MIPs), (bio)chemical sensing and material biocompatibility studies, particularly when low sample volumes are available.
2008
polymer microarrays; MIP; sensors
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/430544
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