A functional genomic approach was undertaken to investigate the role of ANAC102 in resistance to pathogens in Arabidopsis thaliana. The gene product belongs to the NAC protein family, which contains a number of transcription factors unique to plants, associated with development and stress response. No experimental research has apparently been made until now on ANAC102. Previous work in our laboratory indicates that ANAC102 expression is induced by nitric oxide (NO) treatment and in the hypersensitive reaction (HR) of A. thaliana to avirulent Pseudomonas syringae pv. tomato and to Alternaria brassicicola. Arabidopsis Col-0 insertional mutants, carrying a single homozygous T-DNA insertion in the coding region of ANAC102, were obtained. The T-DNA insertion mutants produced undetectable levels of ANAC102 transcript as assessed by Real-Time RT-PCR, even in strongly inducing conditions (treatment with NO donors). These plants were studied to understand whether, and to what extent, resistance could be compromised by the loss-offunction of ANAC102. Experiments performed included observation of macroscopic and microscopic cell death, measurements of bacterial growth in plants, Northern analysis of the expression of pathogenesis related protein-1 (PR-1) and defensin PFD1.2, both in wild type and mutant plants. Results suggest that resistance against P.s. pv. tomato and A. brassicicola in the mutants is not compromised, but also indicate that ANAC102 is an important positive regulator of PR-1 gene expression. Plants overexpressing ANAC102 have also been produced and will be analysed in relation to resistance and hypersensitive cell death.

Functional involvement of the transcription factor ANAC102 in pathogenesis-related protein expression

ZAGO, Elisa Debora;Polverari A.
2005-01-01

Abstract

A functional genomic approach was undertaken to investigate the role of ANAC102 in resistance to pathogens in Arabidopsis thaliana. The gene product belongs to the NAC protein family, which contains a number of transcription factors unique to plants, associated with development and stress response. No experimental research has apparently been made until now on ANAC102. Previous work in our laboratory indicates that ANAC102 expression is induced by nitric oxide (NO) treatment and in the hypersensitive reaction (HR) of A. thaliana to avirulent Pseudomonas syringae pv. tomato and to Alternaria brassicicola. Arabidopsis Col-0 insertional mutants, carrying a single homozygous T-DNA insertion in the coding region of ANAC102, were obtained. The T-DNA insertion mutants produced undetectable levels of ANAC102 transcript as assessed by Real-Time RT-PCR, even in strongly inducing conditions (treatment with NO donors). These plants were studied to understand whether, and to what extent, resistance could be compromised by the loss-offunction of ANAC102. Experiments performed included observation of macroscopic and microscopic cell death, measurements of bacterial growth in plants, Northern analysis of the expression of pathogenesis related protein-1 (PR-1) and defensin PFD1.2, both in wild type and mutant plants. Results suggest that resistance against P.s. pv. tomato and A. brassicicola in the mutants is not compromised, but also indicate that ANAC102 is an important positive regulator of PR-1 gene expression. Plants overexpressing ANAC102 have also been produced and will be analysed in relation to resistance and hypersensitive cell death.
2005
molecular biology; genes; resistance/tolerance/defence of host
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/390239
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